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一、重組抗體表達

重組抗體被廣泛地應用在科學(xué)研究、免疫診斷及治療性抗體領(lǐng)域?？扑固股锷锿瞥鯟ASRATM保證型重 組抗體表達服務(wù)?？扑固股锷镒杂械牟溉楸磉_系統高產(chǎn)量pATX系列載體及馴化293系列和CHO系列細胞， 能實(shí)現全長(cháng)抗體以及多種形式的重組抗體片段的高效分泌表達。無(wú)需免疫，可實(shí)現單克隆抗體及其它 形式重組抗體的高產(chǎn)和可重復生產(chǎn)。

二、服務(wù)內容

CSRA^TM保證型重組抗體表達服務(wù)內容

三、服務(wù)流程

四、武漢科斯坦生物優(yōu)勢

1.科斯坦生物擁有抗體藥物研發(fā)專(zhuān)家和技術(shù)支持團隊，可對您感興趣的抗體講行個(gè)性化的序列優(yōu)化設計，提高重組抗體表達量。全長(cháng)抗體分泌表達量可達100-300mg/L實(shí)現一次表達終身受益。

2.豐富的抗體表達經(jīng)驗和技術(shù)團隊，已經(jīng)成功表達過(guò)多種形式的全長(cháng)抗體(HumanIgG1~4，Mous e IgG2b,MouseIgG2a,RabbitIgG等)抗體片段(scfv，Fab,tandem-scfv，VHH，Fc融合蛋白。 bi-specificantibody等)。

3.科斯坦生物擁有多種服務(wù)平臺，在實(shí)現重組抗體設計表達和純化的同時(shí)，同時(shí)可以實(shí)現抗體的活性驗證。目前已經(jīng)設計完成了多個(gè)嵌合抗體的表達及活性驗證，并對部分人鼠嵌合抗體進(jìn)行人源化設計驗證。真正實(shí)現一站式服務(wù)。

五、案例展示

Recombinant Antibody Production Cases

Expression caseI:scFv

Expression system:Bsubtilis ExpressionSystem

Service Type:Recombinant Protein Production

Conclusion: Total obtained 37mg scFy from 1L scale up purification, purity is about 90%.

Figure 1. Expression result

Figure 2. Target scFv purification profile

Figure 3. Final sample QC

Expression caseⅡ:Bi-specific Antibody

Expression System:Mammalian 293F Expression System

Service Type: Recombinant Antibody Production

Conclusion:Bi-specific antibody A total obtained 17.35mg per liter of culture, purity> 90% Bi-specific antibody B total obtained 16.23mg per liter of culture, purity> 85%.

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression casellI: IgG-like bi-specific antibody

Expression System:Mammalian CHO-S Expression System

Service Type:Recombinant Antibody Production

Conclusion:Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%

Figure 1. Expression result

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression caselII: HH expressed with tag and tag removal

Service Type:Recombinant Antibody Production

Conclusion: Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%

Target protein purification profile in native condition.

Tag cleavage results.

1. Before cleavage sample 2. After cleavage sample

Final sample QC

六、咨詢(xún)與訂購

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